CELLS COLLECTED FROM THE CORNEAL SURFACE IN SJOGREN'S SYNDROME, DRY EYE, AND NORMALS

Title CELLS COLLECTED FROM THE CORNEAL SURFACE IN SJOGREN'S SYNDROME, DRY EYE, AND NORMALS
Author, Co-Author Salih Al-Oliky, Graeme Wilson, Carolyn Begley, Gerald Lowther
Topic
Year
2000
Day
Sunday
Program Number
Poster 81
Room
Northern Hemisphere C-D
Affiliation
Abstract Purpose. The purpose of this study was to determine if the number of cells that can be removed from the epithelial surface is different in dry eye states. Attention was paid to whether cells were nucleated or without nuclei. It was hypothesized that the number of cells would be greater in the dry eye states. Methods. Four Sjogren's syndrome (SS), four dry eye (DE), and four age-matched normals served as subjects. Cells were collected by sequential contact lens cytology, stained with acridine orange and Hoechst, and then evaluated using epifluorescence microscopy at 500X. For counting, cells were categorized as nucleated or enucleated by their ability to stain with Hoechst. Cells without nuclei were described as cell ghosts. Results Nucleated; ghost cells: SS =137+/-56; 554+/-116 DE= 114+/-46; 700+/-244 Normal= 72+/-11;173+/-20 . The number of cells was significantly greater in DE and SS compared with normal (Mann-Whitney U-test, one tail, pUSING THE FLEXLENS PIGGYBACK LENS SYSTEM TO OVERCOME RGP DECENTRATION DUE TO IRREGULAR ASTIGMATISM IN ADVANCED KERATOCONUS
Affiliation of Co-Authors
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