|Title||COMPOSITION OF CELLS SHED FROM THE OCULAR SURFACE|
|Author, Co-Author||Jie Zhou, Carolyn Begley, Graeme Wilson|
Southern Hem III
|Abstract|| PURPOSE. This study determined the composition of cells shed from the ocular surface over the course of one day.
METHODS. Ocular surface cells were collected using the method of contact lens cytology from 10 subjects every 3 hours during one day. Each subject wore NewVues contact lenses in both eyes for 10 minutes. Lenses were then removed with sterile gloves and rinsed with culture medium. Samples were pooled from both eyes of each subject and divided into three equal portions for analysis. Following fixation with acetone and alcohol, one third of each sample was incubated with anti-keratin antibody AE5 (corneal epithelium), one third with anti-keratin antibody AE1 and AE3 (all epithelia), and one third with anti-T200 (bone marrow derived cells). Samples were incubated with fluorescein-conjugated goat anti-mouse antibody and filtered through membranes with 5µm pores. Ethidium homodimer was used as a counterstain. Fluorescein stained cells were counted, followed by staining with toluidine blue to obtain total cell counts.
RESULTS. Over a period of one day, all subjects showed a statistically significant (pCOMPUTER SIMULATION OF RIGID LENS FLUORESCEIN PATTERNS
|Affiliation of Co-Authors|