|Title||COMPOSITION OF CONJUNCTIVAL EPITHELIAL FLAPS|
|Author, Co-Author||Mosaad Alabdulmunem, Johanna Henriksson, Norman Leach, Michael Doughty, William Miller, Jan Bergmanson|
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|Abstract|| PURPOSE: Conjunctival epithelial flaps (CEF) appear to be created by tightly fitted soft contact lenses (CL) manufactured with a sharp edge. The cellular content of these flaps have yet to be investigated. The purpose of this study was to investigate the composition of CEF by utilizing impression cytology.
METHODS: Six subjects, 2 non-lens wearers, 2 contact lens wearers without flaps and 2 contact lens wearers with flaps all wearing Air Optix Night and Day Aqua lenses where enrolled in this study. The flaps were observed in the superior and inferior bulbar conjunctival quadrant approximately 1.0 mm from the limbus. Impression cytology samples were collected from the flap locations of both the left and right eyes from all subjects using Millipore HAWP02500 membrane filters, cut in 5X1 mm rectangles were gently pressed onto the conjunctiva for a few seconds and then removed. Subsequently the filters were fixed in 95% alcohol, stained with hematoxylin, eosin. The filters were viewed with an Olympus IX70 microscope. Measurements of the longest dimension (LONG) and the longest dimension of the nucleus (NUCLONG) were measured on 40 cells from each filter by utilizing NIH Image 1.63.
RESULTS: CEF were formed by multi layers of epithelial and goblet cells but devoid of white blood cells, basement membrane material and stromal tissue. The superficial epithelial cells from the right eyes measured on average 22.5±5.0 µm for the non-lens wearers, 21.5±3.8 µm for the flaps and 26.6±5.2 µm for the CL wearers without flaps. Similar results were obtained with left eyes The NUCLONG measures for the same samples were 9.6±1.3 (non-lens wearer), 9.6±2.0 (flaps), 10.6±2.3 (CL wearers without flaps) The cytoplasm-to-nucleus (LONG-NUCLONG/NUCLONG) ratios for the non-lens wearers were 1.30, for the flaps 1.42 and for the CL wearers without flaps 1.78.
CONCLUSIONS: CEF is an epithelial, non-inflammatory phenomenon consisting of apparently healthy epithelial and goblet cells.
|Affiliation of Co-Authors||Texas Eye Research & Technology Center, UH College of Optometry, Texas Eye Research & Technology Center, UH College of Optometry, Glasgow Caledonian University, Texas Eye Research & Technology Center, UH College of Optometry, Texas Eye Research & Technology Center, UH College of Optometry|