THE EFFECT OF DESICCATION ON SURVIVAL OF MICROORGANISMS IN CONTACT LENS CASE WELLS`

Title THE EFFECT OF DESICCATION ON SURVIVAL OF MICROORGANISMS IN CONTACT LENS CASE WELLS`
Author, Co-Author Marc Ajello, Ida Apirutvarakul, William Long, Jr.
Topic
Year
1996
Day
Sunday
Program Number
Poster 32
Room
Southern Hem I,II
Affiliation
CIBA Vision Corporation
Abstract PURPOSE. Many manufacturers recommend air drying contact lens cases to reduce or eliminate bacteria and fungi colonization. This study investigated various periods of desiccation on experimental microbial films in contact lens case wells. METHOD. New lens cases were inoculated with model biofilms which were prepared using either Aspergillus fumigatus conidia, Pseudomonas aeruginosa or Staphylococcus epidermidis suspended in an organic soil. Inoculum levels of approximately 1,000 colony forming units per milliliter were deposited in each lens case. Sets of lens cases were then allowed to dry overnight, 7 days, and 28 days. After the designated time interval each lens case well was rehydrated with sterile saline and sampled for microbiological enumeration. RESULTS. Recovery of viable organisms of each species tested was seen following overnight desiccation At the seven day time point only S. epidermidis and A. fumigatus were recovered. At the 28-day time interval only A. fumigatus survived. CONCLUSION. In the laboratory setting, not all microorganisms are eliminated by air drying contact lens cases. The contact lens case remains a potential source of contamination following prolonged periods of desiccation. These findings support manufacturers' recommendations for periodic lens case replacement.
Affiliation of Co-Authors CIBA Vision Corporation, CIBA Vision Corporation
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