NEAR UV DAMAGE TO LENS EPITHELIUM AND ANTIOXIDANT PROTECTION

Title NEAR UV DAMAGE TO LENS EPITHELIUM AND ANTIOXIDANT PROTECTION
Author, Co-Author Seymour Zigman, Joanne Schultz, Thurma McDaniel
Topic
Year
1993
Day
Monday
Program Number
Poster 52
Room
Exhibit Hall
Affiliation
Abstract We report here on studies of how (1) the quality and quantity of sunlight UV energy that reaches the lens damage specific biochemical entities in epithelial cells and (2) if antioxidants with known actions reduce this damage. Cultured squirrel and rabbit lens epithelial cells (provided by D. John Reddan, Oakland University) were exposed to known quantities of near-UV radiation (UVA, UVB) in the presence or absence of antioxidants (e.g. alpha-tocopherol, deferoxamine, DABCO). Approximately 3 J/cm^2 of UVA and 4 mJ/cm^2 of UVB reached the cells. The following parameters were compared with controls: cell viability and survival, DNA integrety, actin integrety, and the enzymatic activities of Na-K-ATPase and catalase. The results indicated that sufficient mixed UVA and UVA energy reaches the lens from sunlight to retard growth and survival. It depolymerizes actin, and inactivate catalase and Na-K-ATPase, and thus may be a real factor in cataract formation. The data indicated that alpha-tocopherol, other antioxidants and free radical scavengers protected these intracellular targets from UV damage. Appropriate antioxidants that decrease oxidative lens damage do to UV exposure may also delay age-related changes leading to lens opacities. Research support: NEI (EY 00459); and Research to Prevent Blindness, Inc
Affiliation of Co-Authors
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