QUANTIFICATION OF DEPOSITS ON IN VITRO DEPOSITED SOFT CONTACT LENSES USING VIDEO IMAGE ANALYSIS: CORRELATION BETWEEN VIDEO IMAGE ANALYSIS AND FLUOROM

Title QUANTIFICATION OF DEPOSITS ON IN VITRO DEPOSITED SOFT CONTACT LENSES USING VIDEO IMAGE ANALYSIS: CORRELATION BETWEEN VIDEO IMAGE ANALYSIS AND FLUOROM
Author, Co-Author Thierry Bilbault, Bahram Asgharian, Mary Ingram, Masood Chowhan
Topic
Year
1992
Day
Sunday
Program Number
Poster 18
Room
Great Hall
Affiliation
Abstract Validation studies were conducted during the development of a video image analysis system to ensure the reproducibility and validity of the method designed to objectively assess deposits on soft hydrophilic lenses. A fluorometer was custom designed and built to detect and quantify the amount of dansyl-lysozyme deposited on soft lenses. The sensitivity and linearity of the fluorometric technique were demonstrated and the method was shown to objectively quantify the amount of fluorescent labelled-lysozyme deposited on lenses. Group I and IV lenses were in vitro soiled in a protein deposition model containing dansyl-lysozyme. The pre and post deposited video images of the lenses were recorded and digitized. The extent of lysozyme deposit was determined using the proprietary software. The same lenses were subjected to fluorometric analysis and the amount of deposited lysozyme evaluated. High correlation between the deposit area evaluated by video image analysis and the protein levels determined by the fluorometric method was demonstrated for Soflens (r=0.8705) and Durasoft 3 (r=0.8345) lenses. The video image analysis system developed is a reliable, valid, nondestructive, quantitative and objective method for the measurement of deposits present on in vitro soiled soft contact lenses.
Affiliation of Co-Authors
Outline