|Title||SHORT-TERM EFFECT OF DORZOLAMIDE ON OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY (OCTA) RETINAL VASCULAR FINDINGS IN HEALTHY EYES|
|Author, Co-Author||Carolyn Majcher, Richard Trevino, Wesley Juett, Aneal Dosch, Kelsey Price, William Sponsel, Adreain Henry|
|Topic||Treatment and Management of Posterior Sgmt Disease|
Purpose: To quantify the short-term response of macular retinal vasculature induced by topical dorzolamide using OCTA in normal human eyes.
Methods: OCTA macular cubes (ZEISS AngioPlex®) and IOPs (iCare®) were performed OU on 30 healthy adults. Dorzolamide 2% was then instilled in one eye. lOP and OCTA were repeated at 30 and 60min. 3 and 6mm superficial (3Sc, 6Sc; ILM-IPL), and 3 and 6mm deep (3Dp, 6Dp; IPL–OPL) en-face angiograms were analyzed. Unaltered histograms (0 black-255 white) were used to calculate % pixel densities (PD), or pixel sums within predetermined ranges/total pixels. PD ranges for 3mm angiograms were: background (B) 0-30, capillary (C) 45-70, arteriole (A) 71-130. Ranges for 6mm were: B 0-45, C 60-90, A 91-140. % vessel density (VD = white/total pixels) was done using ImageJ local threshold. Paired 2-tailed ttests were used.
Results: Average age (± SD) was 28.9 ± 6.9 yr. 15 subjects were female and 18 study eyes (SEs) were OD. IOP decreased OU at 30min but continued to fall in SEs resulting in asymmetry at 1hr (13.9 ± 3.0 vs 14.8 ± 2.8, p = 0.02). In SEs, B PD decreased (3Sc: -2.0 ± 3.9, p = 0.01; 3Dp: -2.2 ± 5.4, p = 0.04), while C PD (3Sc: +0.7 ± 1.6, p = 0.02, 3Dp: +0.8 ± 2.1, p = 0.04) and A PD (3Sc: +1.6 ± 3.5, p = 0.03) increased at 30min. In 6Sc and 6Dp scans, PD asymmetry emerged at 30min (6Sc: B p = 0.03, C p = 0.04, A p = 0.02; 6Dp: B p = 0.04, C p = 0.04, A p = 0.002) with lower B PDs and greater C and A PDs in SEs. Only 3Sc C PD increase persisted at 1hr. No PD changes occurred in non-SEs. SE 6Dp and 3Dp VD increases approached significance at 30min.
Conclusion: Dorzolamide induced transient, consistent macular OCTA B PD decrease and C PD, A PD increases suggestive of histogram shifting. This may represent decreased background/increased vascular area or vessel brightening and requires qualification. Alterations did not appear to coincide with max IOP drop.
|Affiliation of Co-Authors||Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry|