SHORT-TERM EFFECT OF DORZOLAMIDE ON OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY (OCTA) RETINAL VASCULAR FINDINGS IN HEALTHY EYES

Title SHORT-TERM EFFECT OF DORZOLAMIDE ON OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY (OCTA) RETINAL VASCULAR FINDINGS IN HEALTHY EYES
Author, Co-Author Carolyn Majcher, Richard Trevino, Wesley Juett, Aneal Dosch, Kelsey Price, William Sponsel, Adreain Henry
Topic Treatment and Management of Posterior Sgmt Disease
Year
2016
Day
Thursday
Program Number
165158
Room
Ballroom A-B
Affiliation
UIW Rosenberg School of Optometry
Abstract

Purpose: To quantify the short-term response of macular retinal vasculature induced by topical dorzolamide using OCTA in normal human eyes.

Methods: OCTA macular cubes (ZEISS AngioPlex®) and IOPs (iCare®) were performed OU on 30 healthy adults. Dorzolamide 2% was then instilled in one eye. lOP and OCTA were repeated at 30 and 60min. 3 and 6mm superficial (3Sc, 6Sc; ILM-IPL), and 3 and 6mm deep (3Dp, 6Dp; IPL–OPL) en-face angiograms were analyzed. Unaltered histograms (0 black-255 white) were used to calculate % pixel densities (PD), or pixel sums within predetermined ranges/total pixels. PD ranges for 3mm angiograms were: background (B) 0-30, capillary (C) 45-70, arteriole (A) 71-130. Ranges for 6mm were: B 0-45, C 60-90, A 91-140. % vessel density (VD = white/total pixels) was done using ImageJ local threshold.  Paired 2-tailed ttests were used.

Results: Average age (± SD) was 28.9 ± 6.9 yr. 15 subjects were female and 18 study eyes (SEs) were OD. IOP decreased OU at 30min but continued to fall in SEs resulting in asymmetry at 1hr (13.9 ± 3.0 vs 14.8 ± 2.8, p = 0.02). In SEs, B PD decreased (3Sc: -2.0 ± 3.9, p = 0.01; 3Dp: -2.2 ± 5.4, p = 0.04), while C PD (3Sc: +0.7 ± 1.6, p = 0.02, 3Dp: +0.8 ± 2.1, p = 0.04) and A PD (3Sc: +1.6 ± 3.5, p = 0.03) increased at 30min. In 6Sc and 6Dp scans, PD asymmetry emerged at 30min (6Sc: B p = 0.03, C p = 0.04, A p = 0.02; 6Dp: B p = 0.04, C p = 0.04, A p = 0.002) with lower B PDs and greater C and A PDs in SEs. Only 3Sc C PD increase persisted at 1hr. No PD changes occurred in non-SEs. SE 6Dp and 3Dp VD increases approached significance at 30min.

Conclusion: Dorzolamide induced transient, consistent macular OCTA B PD decrease and C PD, A PD increases suggestive of histogram shifting. This may represent decreased background/increased vascular area or vessel brightening and requires qualification. Alterations did not appear to coincide with max IOP drop. 

Affiliation of Co-Authors Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry, Rosenberg School of Optometry
Outline