Striking similarities in tau oligomer aggregation and subsequent inflammation between the eye and the brain in Alzheimer disease mouse models.

Title Striking similarities in tau oligomer aggregation and subsequent inflammation between the eye and the brain in Alzheimer disease mouse models.
Author, Co-Author Praveena Gupta, Kelsey English, Ashley Nilson, Julia Gerson, Gibran Khurshid, Thomas Whittle, Diana Castillo-Carranza, Rakez Kayed
Topic Systemic/Ocular Disease
Year
2016
Day
Thursday
Program Number
165152
Room
Ballroom A-B
Affiliation
Abstract

Purpose: Tau oligomers have been shown to initiate the formation of neurotoxic Neurofibrillary tangles (NFT’s) in the brains of Alzheimer’s disease.  Eye being an extension of the brain we propose to investigate if there are parallel findings of the tau oligomer initiated inflammation between the brain and the eyes of the Alzheimer’s mouse models.

Methods: Eyes and brains from wild type, Htau, P301L mouse models were harvested and processed for cryosections. Immunofluorescence was performed using anti-T22 tau oligomer-specific antibody, GFAP, Iba1, antibodies both in brain and retinal sections and images were then captured using confocal fluorescence microscope.

Results: Characteristic tau oligomers were present in the brain and retina of the Htau and P301L transgenic mouse models. Besides the structural similarity of the tau oligomer aggregation, markers for inflammation were noted at the vicinity of the oligomer aggregates in both brain and eyes. Activated glial cells with engulfed tau-oligomers and with co-localization with the anti T22 antibody were found both in the retinal layers and in the brains of the transgenic mouse. Age-matched wild type did not show tau oligomer and/or inflammation in brain or the eyes.

Conclusions: Our findings provide evidence that pathological events triggered in by the tau oligomers in the brain extend in the retina with simultaneous pro-inflammatory events asserting the close association between the brain and the eyes in Alzheimer’s disease mouse models.

 

Affiliation of Co-Authors University of Texas Medical Branch, University of Texas Medical Branch, University of Texas Medical Branch, University of Florida, University of Texas Medical Branch, University of Texas Medical Branch, University of Texas Medical Branch Outline