COTTON WOOL VERSUS CALCIUM ALGINATE SWABS FOR SAMPLING CONJUNCTIVAL MICROBIOTA

Claire Fleming

Abstract

The recovery of ocular isolates from the lower conjunctival fornix for microbiological analysis may be influenced by the collection technique. Calcium alginate (CA) swabs may yield greater numbers of organisms than cotton wool (CW) swabs, but have also been reported to be toxic to some microorganisms. In this study, the recovery of ocular microbiota using CA and CW swabs was compared in a group of 10 asymptomatic and 13 symptomatic patients (6 with bacterial conjunctivitis and 7 with bacterial corneal ulcers). One eye of each subject was sampled by swabbing the nasal and temporal inferior conjunctival fornix separately with either a CA or CW swab. Swabs were immediately immersed in Calgon Ringer's solution and vortexed for 30 seconds; aliquots of solution were then inoculated on to agar plates and incubated under standard conditions. Samples from 3 asymptomatic and 6 symptomatic subjects yielded no bacterial growth. For the other subjects, the CA swabs recovered a significantly greater number of microorganisms than the CW swabs (p ANALYSIS OF RGP CONTACT LENS DEPOSITS

Details

Year: 1992

Program Number: Poster 10

Author Affiliation: n/a

Co-Authors: Savitri Sharma, Jo-Anne Chuck, Sankaridurg Padmaja, KR Lakshmi, Madhukar Reddy

Co-Author Affiliation: n/a

Room: Great Hall