DOES TISSUE WIPING CONTAMINATED DIAGNOSTIC PHARMACEUTICALS REDUCE THE CHANCES OF INSTILLING A CONTAMINATED DROP IN A PATIENT'S EYE?

Ann Katzenberger

Abstract

PURPOSE. Studies have shown that bottle tips and the inside surface of caps of diagnostic pharmaceuticals in clinical use may become contaminated with bacteria. Prior attempts to decrease the frequency of contamination of drops from contaminated bottles have shown an alcohol wipe of the bottle tip to be partially successful. Since wiping a bottle tip with alcohol prior to use may be impractical in a clinical setting, we decided to investigate whether wiping a contaminated bottle tip with a dry tissue might lower the frequency of contaminated drops being obtained. METHOD. Staphylococcus epidermidis bacteria were inoculated onto the tips of 30 bottles of 1% tropicamide ophthalmic solution and 30 bottles of 0.5% proparacaine hydrochloride ophthalmic solution. Bottles were recapped and stored at room temperature for 24 hours. Drops from each of the bottles were dripped on 5% sheepsblood agar plates and incubated at 37 degrees C for 60 hours. One drop from each contaminated bottle was dripped onto sheepsblood agar. Each bottle was then wiped with a dry tissue and two drops were dripped onto sheepsblood agar.

RESULTS. Contaminated drops were obtained from only 40% (12/30) of the bottles with tips which had been contaminated. Wiping the contaminated bottles with a dry tissue decreased the frequency of contamination to 25% (3/12) for the first and second drops from the tropicamide solution and to 34% (4/12) and 17% (2/12) for the first and second drops from the proparacaine solution, respectively.

CONCLUSIONS. A dry tissue wipe of contaminated bottle tips of proparacaine and tropicamide can decrease the frequency of obtaining a contaminated drop of solution from these bottles.

Details

Year: 2001

Program Number: Poster 68

Author Affiliation: University of Houston

Co-Authors: Judith Perrigin, David Perrigin, Roger Boltz

Co-Author Affiliation: University of Houston, University of Houston, University of Houston

Room: Exhibit Hall C