THE EFFECT OF TONICITY AND NUTRIENT LOAD ON THE SURVIVAL AND GROWTH OF THE ACANTHAMOEBA IN VITRO

Yihua Wang

Abstract

Acanthamoeba keratitis is an uncommon but severe corneal infection that is more common among contact lens wearers than among non contact lens wearers. The mechanism of pathogenesis of amoebic keratitis remains unexplained. To examine whether environmental factors such as tonicity and nutrient load play a role in the survivability and ultimate infectivity of the Acanthamoeba, human corneal isolates of [italics]A. castellanii and [italics]A. polyphaga trophozoites were cultured in two different media--one with and another without nutrients. The tonicity of the media was varied from 50 to 3438 mOsm/L. At the time zero and at pre-set intervals, aliquots of the test media containing the amoebae were recovered and assayed to assess the ratio of trophozoites and cysts in the suspensions. The results showed that the survival rate of the amoebae was highest in the low tonicity solutions(VISUAL IMPAIRMENT IN NORMOTENSIVE GLAUCOMA

Details

Year: 1992

Program Number: Poster 8

Author Affiliation: n/a

Co-Authors: Jan Bergmanson, Michael Osato

Co-Author Affiliation: n/a

Room: Great Hall