EFFECTS OF ULTRAVIOLET RADIATION ON THE SHEDDING RATE OF THE CORNEAL EPITHELIUM IN VITRO

Hong Ren

Abstract

Histological studies following ultraviolet radiation have shown that cell shedding by the epithelium is increased. To understanding the basic mechanisms and clinical implications of this effect it is necessary to know the latency of this increase.Rabbit corneas were excised and perfused in pairs. The epithelial surfaces were bathed with Basic Tear Solution at 304mOsm/kg and pH 7.4, and the endothelial surfaces with Glutathione-Bicarbonate Ringer solution. One cornea in each pair was irradiated with a suprathreshold dose of UVB (290-310nm, 0.25J/cm2), while the other cornea served as a control. Every 50 min, the cells in the epithelial bathing solutions were stained with acridine orange and counted using fluorescence microscopy. The shedding was compared by subtracting the control cornea from the UV cornea to obtain a difference in cells shed. There are significant differences in cell shedding rates at 200,250,300,350, and 400 min(ANOVA, pCONTACT LENSES AND THE ETIOLOGY OF PTERYGIUM FORMATION

Details

Year: 1992

Program Number: Poster 31

Author Affiliation: n/a

Co-Authors: Graeme Wilson

Co-Author Affiliation: n/a

Room: Great Hall